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Fluorescent Dyes Used in STED Microscopy

Dye name
(Manufacturer / Distributor)
Exc. Wavelength STED
Wavelength
Spatial Resol.
(direction)
Reference(s)
ATTO 425 440 532 70 - 80 nm (xy)

B. Rankin et al., Opt. Lett. 33, 2491 (2008)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: 130 ps
STED Pulse Lentgh: 1 ns
Repetition Rate: 60 kHz
Average STED Power: 24 - 162 µW
Peak Irradiance: 0.4 - 2.7 nJ
Pulse Energy: 500 MW/cm2
Abberrior STAR 440SX 440 590

--

Additional Information

Distributor/Manufacturer: Abberior GmbH
Excitation Pulse Length: --
STED Pulse Lentgh: --
Repetition Rate: --
Mn doped ZnSe Qdots 440 676 45 nm (xy)

S.Irvine et al., Angew. Chem. Int. Ed. 47, 2685 (2008)

Additional Information

Excitation Pulse Length: < 90 ps
STED Pulse Lentgh: cw
Repetition Rate: cw
Peak Irradiance: 9 MW/cm2
ATTO 532 470 603 <25 nm (xy)

G. Donnert et al., Proc. Natl. Acad. Sci. USA 103, 11440 (2006)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: 80 ps
STED Pulse Lentgh: 280 ps
Repetition Rate: 250 kHz
Average STED Power: 0.5 mW
Pulse Energy: 2 nJ
ATTO 532 470 615 66 - 72 nm (xy)

K. Willig et al., Nature 440, 935 (2006)
J. Sieber et al., Biophys. J. 90, 2843 (2006)
K. Willig et al., New J. Phys. 8, 106 (2006)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: 100 ps
STED Pulse Lentgh: 200 ps
Repetition Rate: 80 MHz
Average STED Power: 14 - 18 mW
Alexa Fluor 488 488 592 < 60 nm (xy)

G. Moneron et al., Opt. Expr. 18, 1302 (2010)

Additional Information

Distributor/Manufacturer: Invitrogen Corp.
Excitation Pulse Length: cw
STED Pulse Lentgh: cw
Repetition Rate: cw
Average STED Power: < 600 mW
Chromeo 488 488 592 < 60 nm (xy)

G. Moneron et al., Opt. Expr. 18, 1302 (2010)

Additional Information

Distributor/Manufacturer: Actif Motif Inc.
Excitation Pulse Length: cw
STED Pulse Lentgh: cw
Repetition Rate: cw
Average STED Power: < 600 mW
Oregon Green 488 488 592 < 60 nm (xy)

G. Moneron et al., Opt. Expr. 18, 1302 (2010)

Additional Information

Distributor/Manufacturer: Invitrogen Corp.
Excitation Pulse Length: cw
STED Pulse Lentgh: cw
Repetition Rate: cw
Average STED Power: < 600 mW
FITC 488 592 < 60 nm (xy)

G. Moneron et al., Opt. Expr. 18, 1302 (2010)

Additional Information

Excitation Pulse Length: cw
STED Pulse Lentgh: cw
Repetition Rate: cw
Average STED Power: < 600 mW
DY-495 488 592 < 60 nm (xy)

G. Moneron et al., Opt. Expr. 18, 1302 (2010)

Additional Information

Distributor/Manufacturer: Dyomics GmbH
Excitation Pulse Length: cw
STED Pulse Lentgh: cw
Repetition Rate: cw
Average STED Power: < 600 mW
Citrine 488 592 ~ 60 nm (xy)

B. Hein et al., Proc. Natl. Acad. Sci. USA 105, 14271 (2008)

Additional Information

Excitation Pulse Length: cw
STED Pulse Lentgh: cw
Repetition Rate: cw
Average STED Power: 112 mW
Peak Irradiance: 62 MW/cm2
ATTO 532 488 600 <40 nm (xy)

L. Meyer et al., Small 4, 1095 (2008)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: 120 ps
STED Pulse Lentgh: 200 ps
Repetition Rate: 250 kHz
Average STED Power: --
Peak Irradiance: 1.1 GW/cm2
Pulse Energy: --
Chromeo 488 488 602 ~ 30 nm (xy)

L. Meyer et al., Small 4, 1095 (2008) (Supporting Material)

Additional Information

Distributor/Manufacturer: Actif Motif Inc.
Excitation Pulse Length: 140 ps
STED Pulse Lentgh: 200 ps
Repetition Rate: 250 kHz
Average STED Power: 0.6 mW
ATTO 532 488 615 60 - 70 nm (xy)

D. Fitzner et al., EMBO J. 25, 5037 (2006); R. Kellner, Neurosci. 144, 135 (2007)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: 100 ps
STED Pulse Lentgh: 200 ps
Repetition Rate: 80 MHz
Average STED Power: 16 mW
DY-485XL 488 647 40 - 45 nm (xyz)

R. Schmidt et al., Nat. Meth. 5, 539 (2008)

Additional Information

Distributor/Manufacturer: Dyomics GmbH
Excitation Pulse Length: < 100 ps
STED Pulse Lentgh: ~ 200 ps
Repetition Rate: 72 MHz
Average STED Power: (20 + 3) mW
GFP 490 575 ~ 70 nm (xy)

K. Willig et al., Nat. Meth. 3, 721 (2006)

Additional Information

Excitation Pulse Length: 100 ps
STED Pulse Lentgh: 200 ps
Repetition Rate: 80 MHz
Average STED Power: 7.2 mW
Citrine 490 598 48 nm (xy)

B. Hein et al., Proc. Natl. Acad. Sci. USA 105, 14271 (2008)

Additional Information

Excitation Pulse Length: 100 ps
STED Pulse Lentgh: 300 ps
Repetition Rate: 80 MHz
Average STED Power: 36 mW
Peak Irradiance: 807 MW/cm2
Pulse Energy: 0.45 nJ
YFP 490 598 ~ 70 nm (xy)

U. V. Nägerl et al., Proc. Natl. Acad. Sci. USA 105, 18982 (2008)

Additional Information

Excitation Pulse Length: 100 ps
STED Pulse Lentgh: 300 ps
Repetition Rate: 80 MHz
Average STED Power: 18 mW
Peak Irradiance: 400 MW/cm2
Pulse Energy: 0.23 nJ
Abberior CAGE 500 500 600

V. N. Belov et al., Angew. Chem. Int. Ed. 49, 3520?3523 (2010)
G. Y. Mitronova et al., Chem. Eur. J. 16, 4477?4488 (2010)

Additional Information

Distributor/Manufacturer: Abberior GmbH
Excitation Pulse Length: --
STED Pulse Lentgh: --
Repetition Rate: --
Abberior STAR 512 512 605

G. Y. Mitronovaet al., Chem. Eur. J. 16, 4477-4488 (2010)
S. M. Polyakova et al., Eur. J. Org. Chem. 2009, 5162 (2009)

Additional Information

Distributor/Manufacturer: Abberior GmbH
Excitation Pulse Length: --
STED Pulse Lentgh: --
Repetition Rate: --
ATTO 565 532 640 30 - 40 nm (xy)

D. Wildanger et al., Opt. Expr. 16, 9614 (2008)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: ~ 90 ps
STED Pulse Lentgh: ~ 90 ps
Repetition Rate: 1 - 2 MHz
Average STED Power: --
Sulfonated & rigidized rhodamine derivatives 532 640 < 90 nm (xy)

V. Boyarskiy et al., Chem. Eur. J. 14, 1784 (2008)

Additional Information

Excitation Pulse Length: 100 ps
STED Pulse Lentgh: ~ 300 ps
Repetition Rate: 80 MHz
Peak Irradiance: 40 MW/cm2
ATTO 565 532 647 ~ 60 nm (xy)

K. Willig et al., Nat. Meth. 4, 915 (2007)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: cw
STED Pulse Lentgh: cw
Repetition Rate: cw
Average STED Power: 114 mW
NK51 532 647 40 - 45 nm (xyz)

R. Schmidt et al., Nat. Meth. 5, 539 (2008)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: < 100 ps
STED Pulse Lentgh: ~ 200 ps
Repetition Rate: 72 MHz
Average STED Power: (20 + 3) mW
Nitrogen vacancies in diamond 532 775 6 nm (xy)

Rittweger et al., Nat. Photon. 3, 144 (2009)

Additional Information

Excitation Pulse Length: 60 ps
STED Pulse Lentgh: 3.2 ns
Repetition Rate: 8 MHz
Average STED Power: 850 mW
Peak Irradiance: 8.6 GW/cm2
Pulse Energy: 106 nJ
MR 121 SE 532 793 ~ 50 nm (z)

M. Dyba et al., Nat. Biotech. 21, 1303 (2003)

Additional Information

Distributor/Manufacturer: Roche Diagnostics
Excitation Pulse Length: 10 ps
STED Pulse Lentgh: 107 ps
Repetition Rate: 76 MHz
Average STED Power: 10.4 mW
Abberior CAGE 552 552 655

V. N. Belov et al., Angew. Chem. Int. Ed. 49, 3520?3523 (2010)

Additional Information

Distributor/Manufacturer: Abberior GmbH
Excitation Pulse Length: --
STED Pulse Lentgh: --
Repetition Rate: --
Pyridine 2 / LDS 722 554 745 44 nm (z)

M. Dyba et al., New. J. Phys. 7, 134 (2005)

Additional Information

Distributor/Manufacturer: Exciton, Radiant Dyes GmbH
Excitation Pulse Length: 250 fs
STED Pulse Lentgh: 50 - 200 ps
Repetition Rate: 76 MHz
Average STED Power: 12.2 mW
RH 414 554 745 30 nm (z)

M. Dyba, S.W. Hell, Phys. Rev. Lett. 88, 163901 (2002)

Additional Information

Distributor/Manufacturer: Biotium, Inc.
Excitation Pulse Length: 250 fs
STED Pulse Lentgh: 13 ps
Repetition Rate: 76 MHz
Average STED Power: 8.78 mW
Pyridine 2 / LDS 722 554 760 33 nm (z)

M. Dyba, S.W. Hell, Phys. Rev. Lett. 88, 163901 (2002)

Additional Information

Distributor/Manufacturer: Exciton, Radiant Dyes GmbH
Excitation Pulse Length: 250 fs
STED Pulse Lentgh: 13 ps
Repetition Rate: 76 MHz
ATTO 590 570 690 30 - 40 nm (xy)

D. Wildanger et al., Opt. Expr. 16, 9614 (2008)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: ~ 90 ps
STED Pulse Lentgh: ~ 90 ps
Repetition Rate: 1 MHz
Average STED Power: 1.4 - 2.4 mW
Pulse Energy: 1.4 - 2.4 nJ
ATTO 590 570 690 20 nm (xy), 45 nm (xy),
108 nm (z)

D. Wildanger et al., J. Microsc. 236, 35 (2009)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: ~ 90 ps
STED Pulse Lentgh: ~ 90 ps
Repetition Rate: 1 MHz
Average STED Power: 3.8 mW
Pulse Energy: 3.8 nJ
Alexa 594 570 690

D. Wildanger et al., J. Microsc. 236, 35 (2009)

Additional Information

Distributor/Manufacturer: Invitrogen Corp.
Excitation Pulse Length: ~ 90 ps
STED Pulse Lentgh: ~ 90 ps
Repetition Rate: 1 MHz
Average STED Power: 3.8 mW
Pulse Energy: 3.8 nJ
ATTO 594 570 690

D. Wildanger et al., J. Microsc. 236, 35 (2009)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: ~ 90 ps
STED Pulse Lentgh: ~ 90 ps
Repetition Rate: 1 MHz
Average STED Power: 3.8 mW
Pulse Energy: 3.8 nJ
DyLight 594 570 690

D. Wildanger et al., J. Microsc. 236, 35 (2009)

Additional Information

Distributor/Manufacturer: Thermo Scientific
Excitation Pulse Length: ~ 90 ps
STED Pulse Lentgh: ~ 90 ps
Repetition Rate: 1 MHz
Average STED Power: 3.8 mW
Pulse Energy: 3.8 nJ
ATTO 633 630 735 30 - 40 nm (xy)

D. Wildanger et al., Opt. Expr. 16, 9614 (2008)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: ~ 90 ps
STED Pulse Lentgh: ~ 90 ps
Repetition Rate: 1 - 2 MHz
Average STED Power: 1.4 - 2.4 mW
Pulse Energy: 1.4 - 2.4 nJ
ATTO 633 635 750 40 nm (xy)

A. Punge et al., Micr. Res. Techn. 71, 644 (2008)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: 100 ps
STED Pulse Lentgh: ~ 200 ps
Repetition Rate: 76 MHz
Average STED Power: --
Pulse Energy: ~ 1.5 nJ
ATTO 647N 635 750 ~ 50 nm (xy)

K. Willig et al., Nat. Meth. 4, 915 (2007)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: cw
STED Pulse Lentgh: cw
Repetition Rate: cw
Average STED Power: 423 mW
ATTO 647N 635 750 <40 nm (xy)

L. Meyer et al., Small 4, 1095 (2008)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: 120 ps
STED Pulse Lentgh: 200 ps
Repetition Rate: 76 MHz
Peak Irradiance: 1.2 GW/cm2
Abberior STAR 635 635 750

Kolmakov et al., Chem. Eur. J. 16, 158 (2010)
Bückers et al., Opt. Expr. 19, 3130 (2011)

Additional Information

Distributor/Manufacturer: Abberior GmbH
Excitation Pulse Length: --
STED Pulse Lentgh: --
Repetition Rate: --
JA 26 635 775 16 nm (x)

V. Westphal, S.W. Hell, Phys. Rev. Lett. 94, 143903 (2005)

Additional Information

Distributor/Manufacturer: K.H. Drexhage, Siegen University
Excitation Pulse Length: 68 ps
STED Pulse Lentgh: 300 ps
Repetition Rate: 76 MHz
Peak Irradiance: 800 MW/cm2
ATTO 647N 635 780 ~ 65 nm (xy)

G. Donnert et al., Biophys. J.: Biophys. Lett., L67 (2007)
V. Westphal et al., Science 320, 247 (2008)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: 100 ps
STED Pulse Lentgh: 300 ps
Repetition Rate: 250 kHz
Peak Irradiance: 700 MW/cm2
JA 26 635 780 47 nm (xy)

V. Westphal et al., J. Phys. B: At. Mol. Opt. Phys. 38, S695 (2005)

Additional Information

Distributor/Manufacturer: K.H. Drexhage, Siegen University
Excitation Pulse Length: 68 ps
STED Pulse Lentgh: 300 ps
Repetition Rate: 80 MHz
Average STED Power: 90 - 100 mW
Pulse Energy: 1.13 - 1.25 nJ
JA 26 635 781 40 nm (x)

V. Westphal et al., Appl. Phys. B. 77, 377 (2003)

Additional Information

Distributor/Manufacturer: K.H. Drexhage, Siegen University
Excitation Pulse Length: 68 ps
STED Pulse Lentgh: 303 ps
Repetition Rate: 40 MHz
Average STED Power: 10.1 mW
Pulse Energy: 0.25 nJ
JA 26 637 778 120 - 140 nm (xy)

V. Westphal et al., Appl. Phys. Lett. 82, 3125 (2003)

Additional Information

Distributor/Manufacturer: K.H. Drexhage, Siegen University
Excitation Pulse Length: 54 ps
STED Pulse Lentgh: 303 ps
Repetition Rate: 40 MHz
ATTO 565 1060 676 ~ 70 nm (xy)

G. Moneron, S.W. Hell, Opt. Expr. 17, 14567 (2009)

Additional Information

Distributor/Manufacturer: ATTO-TEC GmbH
Excitation Pulse Length: 300 fs@117 MHz, 10 mW
STED Pulse Lentgh: cw
Repetition Rate: cw
Average STED Power: 200 mW

Last modified: 02/13/2012