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Throughout the 20th century, it was widely accepted that a light microscope relying on propagating light waves and conventional optical lenses could not discern details that were much finer than about half the wavelength of light, or 200-400 nm, due to diffraction.
Stefan Hell and his team have shattered what was regarded an absolute limit of microscopic resolution. For his development of STED (STimulated Emission Depletion) microscopy, the first light-focusing microscope with a resolution at the nanoscale, Stefan Hell was awarded the Nobel Prize in Chemistry in 2014.
Recently, a fresh look at the basic physical principles underlying nanoscopy has spawned powerful new concepts. In particular, MINFLUX imaging (nanoscopy with MINimal photon FLUXes) has been pioneered in the Hell Labs, obtaining the ultimate (super)resolution: the size of a molecule (~1 nm). At present, MINFLUX and related concepts are explored to create unprecedented measurement capabilities for the life sciences and beyond.
Stefan Hell is a director at both the Max Planck Institute for Biophysical Chemistry in Göttingen and the Max Planck Institute for Medical Research in Heidelberg, Germany.
He is credited with having conceived, validated and applied the first viable concept for overcoming Abbe’s diffraction-limited resolution barrier in a light-focusing fluorescence microscope. For this accomplishment he has received numerous awards, including the 2014 Kavli Prize in Nanoscience and the Nobel Prize in Chemistry.
Stefan Hell received his doctorate (1990) in physics from the University of Heidelberg. From 1991 to 1993 he worked at the European Molecular Biology Laboratory, followed by stays as a senior researcher at the University of Turku, Finland, between 1993 and 1996, and as a visiting scientist at the University of Oxford, England, in 1994. In 1997 he was appointed to the MPI for Biophysical Chemistry in Göttingen as a group leader, and was promoted to director in 2002. From 2003 to 2017 he also led a research group at the German Cancer Research Center (DKFZ). Hell holds honorary professorships in physics at the Universities of Heidelberg and Göttingen.
Stefan Hell’s Autobiography at nobelprize.org | Stefan Hell’s Nobel Lecture (Video)
Schnorrenberg, S. , Ghareeb, H.; Frahm, L.; Grotjohann, T.; Jensen, N.; Teichmann, T.; Hell, S.W.; Lipka, V.; Jakobs, S. (2020): "Live-cell RESLOFT nanoscopy of transgenic Arabidopsis thaliana". Wiley , in early view. view
Pape, J. K. , Stephan, T., Balzarotti, F., Büchner, R., Lange, F., Riedel, D., Jakobs, S., Hell, S.W. (2020): "Multicolor 3D MINFLUX nanoscopy of mitochondrial MICOS proteins". PNAS 117, 20607-20614. view
Bucevicius, J. , Kostiuk, G.; Gerasimaite, R.; Gilat, T.; Lukinavicius, G. (2020): "Enhancing the biocompatibility of rhodamine fluorescent probes by a neighbouring group effect". RSC Chemical Science 11, 7313-7323. view
Stephan, T. , Brüser, C., Deckers, M., Steyer, A.M., Balzarotti, F., Barbot, M., Behr, T.S., Heim, G., Hübner, W., Ilgen, P., Lange, F., Pacheu-Grau, D., Pape, J.K., Stoldt, S., Huser, T., Hell, S.W., Möbius, W., Rehling, P., Riedel, D., Jakobs, S. (2020): "MICOS assembly controls mitochondrial inner membrane remodeling and crista junction redistribution to mediate cristae formation". The EMBO Journal 39, 1-24. view
Belov, V. N. , Stoldt, S., Rüttger, F., John, M., Seikowski, J., Schimpfhauser, J., Hell, S.W. (2020): "Synthesis of Fluorescent Jasplakinolide Analogues for Live-Cell STED Microscopy of Actin". The Journal of Organic Cemistry , 7267-7275. view
Gwosch, K. C. , J. K. Pape, F. Balzarotti, P. Hoess, J. Ellenberg, J. Ries, S. W. Hell (2020): "MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells". Nature Meth. 17, 217-224. view
Fomin, M. , Seikowski, J., Belov, V. N., Hell, S. W. (2020): "Negatively Charged Red-Emitting Acridine Dyes for Facile Reductive Amination, Separation, and Fluorescent Detection of Glycans Maksim A.". Anal. Chem. 92, 5329 - 5336. view
Savicheva, E.A. , Mitronova, G.Y., Thomas, L., Böhm, M.J., Seikowski, J., Belov, V.N., Hell, S.W. (2020): "Negatively Charged Yellow-Emitting 1-Aminopyrene Dyes for Reductive Amination and Fluorescence Detection of Glycans". Angew. Chem. Int. Ed. 59, 5505 - 5509. view
Gerasimaite, R. , Seikowski, J., Schimpfhauser, J., Kostiuk, G., Gilat, T., D'Este, E., Schnorrenberg, S., Lukinavicius, G. (2020): "Efflux pump insensitive rhodamine-jasplakinolide conjugates for G- and F-actin imaging in living cells". Organic Biomolecular Chemistry , 2929-2937. view
Uno, K. , M.L. Bossi, V.N. Belov, M. Irie, S.W. Hell (2020): "Multicolour fluorescent "sulfide-sulfone" diarylethenes with high photo-fatigue resistance". Chemical Communications 56, 2198-2201. view
Gregor, C. (2020): "Bioluminescent Imaging of Single Bacterial Cells Using an Enhanced ilux Operon". Steven Ripp (ed.), Bioluminescent Imaging Chapter 4, 42-52. view
Göttfert, F. , T. Pleiner, J. Heine, V. Westphal, D. Görlich, S. J. Sahl, S. W. Hell (2017): "Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent". PNAS 114, 2125-2130. view
Balzarotti, F. , Y. Eilers, K. C. Gwosch, A. H. Gynna, V. Westphal, F. D. Stefani, J. Elf, S. W. Hell (2017): "Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes". Science 355, 606-612. view
Chojnacki, J. , T. Staudt, B. Glass, P. Bingen, J. Engelhardt, M. Anders, J. Schneider, B. Müller, S. W. Hell, H.-G. Kräusslich (2012): "Maturation-Dependent HIV-1 Surface Protein Redistribution Revealed by Fluorescence Nanoscopy". Science 338, 524 - 528. view
Berning, S. , K. I. Willig, H. Steffens, P. Dibaj, S. W. Hell (2012): "Nanoscopy in a Living Mouse Brain". Science 335, 551. view
Grotjohann, T. , I. Testa, M. Leutenegger, H. Bock, N. T. Urban, F. Lavoie-Cardinal, K. I. Willig, C. Eggeling, S. Jakobs, S. W. Hell (2011): "Diffraction-unlimited all-optical imaging and writing with a photochromic GFP". Nature 478, 204 - 208. view
Hell, S. W. (2007): "Far-Field Optical Nanoscopy". Science 316, 1153-1158. view
Willig, K. I. , R. R. Kellner, R. Medda, B. Hein, S. Jakobs, S. W. Hell (2006): "Nanoscale resolution in GFP-based microscopy". Nature Meth. 3, 721-723. view
Westphal, V. , S. W. Hell (2005): "Nanoscale Resolution in the Focal Plane of an Optical Microscope". Phys. Rev. Lett. 94, 143903 (4 pp.). view
Klar, T. A. , S. Jakobs, M. Dyba, A. Egner, S. W. Hell (2000): "Fluorescence microscopy with diffraction resolution limit broken by stimulated emission". PNAS 97, 8206-8210. view
Hell, S. W. , J. Wichmann (1994): "Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy". Opt. Lett. 19, 780-782. view